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TelePi is a camera system based on the Raspberry Pi Zero v1.3 with its High Quality camera module. TelePi functions as a telepathology camera system as well as a standard color microscope camera. To capture a larger field of view, overlapping single images of a hematoxylin and eosin-stained, formalin-fixed, paraffin-embedded ureter specimen were taken (with approximately a 10% overlap) in 20X using an Olympus BX41 microscope (Olympus, Tokyo, Japan). Before each image was taken, any necessary adjustment in the focus was performed, so that the image stayed sharp. Afterwards, the images were stitched together using the bundled Fiji plugin Stitching v1.2 (Preibisch, Saalfeld et al. 2009). The original single images were saved as JPEGs as captured by the camera without editing. The stitched final output is included in the dataset.
Formalin-fixed, paraffin-embedded hematoxylin and eosin tissue section from a ureter specimen.
stitching, telepathology, fiji, raspberry pi
stitching, telepathology, fiji, raspberry pi
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