Description of Buttiauxella agrestis K33T6 Cells are Gram­stain­negative, flagellated and coccus shaped. Colonies are circular, convex, entire and beige colored on TSA medium after 48 h incubation at 30℃. Cells reduce nitrate to nitrite and are positive for arginine dihydrolase, β ­glucosidase, β ­galactosidase, alkaline phosphatase, leucine arylamidase, valine arylamidase, trypsin, acid phosphatase, naphthol­AS­BI­phosphohydrolase, β ­galactosidase, α ­glucosidase, β ­glucosidase, N ­acetyl­ β ­glucosaminidase (API 20NE/ZYM). Assimilate D­glucose, L­arabinose, D­mannose, D­mannitol, N ­acetyl­D­glucosamine, D­maltose, gluconate, malate, citrate, phenyl­acetate, salicin, D­melibiose, L­fucose, L­histidine, 2­ketogluconate, 4­hydroxy­benzoate, L­proline, L­rhamnose, D­ribose, D­sucrose, malonate, acetate, lactate, L­alanine, 5­ketogluconate, L­serine (API 20NE/ ID 32GN) and utilize dextrin, D­maltose, D­trehalose, D-cellobiose, gentiobiose, D-turanose, stachyose, D-raffinose, α ­D­lactose, D­melibiose, β ­D­glucoside, D­salicin, N ­glucosamine, β ­mannosamine, N ­galactosamine, neuraminic acid, α ­D­glucose, D­mannose, D­fructose, D­galactose, 6­methyl­glucose, D­fucose, L­fucose, L­rhamnose, inosine, D­sorbitol, D­mannitol, glycerol, D­glucose, D­fructose, D­serine, glycyl­L­proline, L­alanine, L­aspartic acid, L­glutamic acid, L­histidine, L­serine, pectin, galacturonic acid, galactonic lactone, D­gluconic acid, D­glucuronic acid, glucuronamide, mucic acid, quinic acid, methyl pyruvate, L­lactic acid, citric acid, L­malic acid, succinic acid, α ­butyric acid and acetic acid as sole carbon sources (Biolog GEN III). Strain K 33 T6 (= NIBRBAC000503076) was isolated from the gut of Korean dark chub (Nipponocypris koreanus) at Hwasun, Jeollanam Province, Korea. The DNA G + C content of the type strain of the species is 47-51 mol %.

Published as part of Lee, Jae-Yun, Jeong, Yun-Seok, Kim, Pil Soo & Bae, Dong-Wook Hyun and Jin-Woo, 2021, Isolation and identification of 18 unrecorded prokaryotic species from the intestinal tracts of aquatic animals in Korea, pp. 1-11 in Journal of Species Research 10 (1) on page 3, DOI: 10.12651/JSR.2021.10.1.001, http://zenodo.org/record/8119987