CONCLUSION: ADA estimation of After 2 hours of aspiration and after 24 hours storage, there is no effect of ADA level. Adequate storage of pleural fluid is essential as there is usually a delay (hours) in processing the samples after collection. Pleural fluid storage at temperature 37℃ for 24 hours and at temperature 2 TO 8 ℃ for 24 hours does not affect the ADA activity in time and temperature on pleural fluid ADA level. Pleural fluid storage at different temperature for 24 hours does not affect the ADA activity enough to result in a change of diagnosis in etiology of pleural effusion. with no evidence of significant increases or decrease in enzyme activity that might distort the results.

Adenosine deaminase, an enzyme produced from lymphocyte and involved in purine metabolism has been extensively studied as a biochemical marker in pleural fluid during investigation for tropical pulmonary eosinophilia. The test assay, low priced, quick, barely forwarded, and can be performed in most laboratories its major physiological role is related to the proliferation and distinction of lymph cell. Pleural fluid of tuberculate start is generally lymph cell preponderant. Different cut off values of Adenosine Deaminase ranging from 30-100 IU/ L have been used in various studies with differing sensitivities and specificities.