During phytopathological surveys plant samples with grapevine leaf roll virus disease symptoms were collected from Salyan, Ismayilli and Absheron regions. Detection of Grapevine leafroll-associated virus 3 (GLRaV-3) was detected in the collected symptomatic leaves using serologycal and molecular methods. At the same time, activities of some enzymes linked to malate and nitrogen metabolism such as malate dehydrogenase, aspartate aminotransferase and alanine aminotransferase and changes in the content of glycine betaine, soluble proteins, relative water content and dry biomass were also investegeted. It was found that activies of NAD-MDH were increased during patogenesis compared to healthy plants and the activies of two transferazes were reduced. It was also determined that virus infection caused an increase of glycine betaine, content of soluble proteins, relative water content and decrease of dry biomass amount.