Simulated query reads used for synthetic benchmarks in Metabuli publication. Prokaryote Subspecies Inclusion Test Illumina paired-end short reads Simulated using Mason2 based on NovaSeq 6000's error rates. prokaryote_inclusion_reads_1.fna.gz prokaryote_inclusion_reads_2.fna.gz PacBio HiFi long reads (Sequel II CCS) Multi-pass reads were simulated using PBSIM3 based on Sequel Il's error profile. CCS reads were generated using PacBio's CCS-read tool. prokaryote_inclusion_sequel_ccs.fq.gz PacBio Sequel II long reads Simulated using PBSIM3 based on Sequel Il's error profile. prokaryote_inclusion_sequel.fq.gz ONT long reads Simulated using PBSIM3 based on ONT's error profile. prokaryote_inclusion_ont.fq.gz Prokaryote Subspecies exclusion Test Illumina paired-end short reads Simulated using Mason2 based on NovaSeq 6000's error rates. prokaryote_ss-exclusion_reads_1.fna.gz prokaryote_ss-exclusion_reads_2.fna.gz PacBio HiFi long reads (Sequel II CCS) Multi-pass reads were simulated using PBSIM3 based on Sequel Il's error profile. CCS reads were generated using PacBio's CCS-read tool. prokaryote_ss-exclusion_sequel_ccs.fq.gz PacBio Sequel II long reads Simulated using PBSIM3 based on Sequel Il's error profile. prokaryote_ss-exclusion_sequel.fq.gz ONT long reads Simulated using PBSIM3 based on ONT's error profile. prokaryote_ss-exclusion_ont.fq.gz Prokaryote Species Exclusion Test Illumina paired-end short reads Simulated using Mason2 based on NovaSeq 6000's error rates. prokaryote_exclusion_reads_1.fna.gz prokaryote_exclusion_reads_2.fna.gz PacBio HiFi long reads (Sequel II CCS) Multi-pass reads were simulated using PBSIM3 based on Sequel Il's error profile. CCS reads were generated using PacBio's CCS-read tool. prokaryote_sp-exclusion_sequel_ccs.fq.gz PacBio Sequel II long reads Simulated using PBSIM3 based on Sequel Il's error profile. prokaryote_sp-exclusion_sequel.fq.gz ONT long reads Simulated using PBSIM3 based on ONT's error profile. prokaryote_exclusion_ont.fq.gz