Objective: To analyze and compare the results obtained from the Bolton Total and Partial analysis, in the specific orthodontic software in comparison with the results obtained from the open access software. Materials and Methods: Data from 49 people who met the exclusion and inclusion criteria were evaluated. The mesiodistal measurements of the dental pieces of the participants were taken in the following programs: NemoCast, MeshMixer and 3D Slicer, after which the calculation of the Bolton Total and Bolton Partial analysis was performed, the data were entered into a database, and then comparative tables were made in the excel program, and the results between the three programs were directly evaluated. Results: According to the direct True or False analysis method, the tables and graphs presented show that there is a discrepancy between the results of the NemoCast, MeshMixer and 3D Slicer programs, with the latter two having the closest values, and the values with the greatest difference belonging to NemoCast and MeshMixer. Conclusion: The results show that the analysis of the 3 software programs shows that the averages present similar values with a result higher than 78% for the 3 cases. However, there are differences in the review of the individual results and comparisons of the records one by one and this is due to the data entry procedure.