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In-Vitro Antimicrobial and Anticancer Properties of Green Synthesized Gold Nanoparticles Using Securinega leucopyrus Leaves Extract

Authors: Gadepaka, Ravi;

In-Vitro Antimicrobial and Anticancer Properties of Green Synthesized Gold Nanoparticles Using Securinega leucopyrus Leaves Extract

Abstract

ABSTRACT The aqueous leaves extract obtained was investigated for the preparation of gold nanoparticles. The obtained AuNPs were characterized by UV–Visible spectroscopy, FTIR, DLS and Zeta potential analysis. Results indicated that the green synthesized AuNPs showed good antibacterial effect against gram positive, gram negative and exhibited % viability of 45.717 and % of cytotoxicity 54.282 on breast cancer (MCF-7) at a maximum concentration of 100 mg/ml. Keywords: Antibacterial, Securinega leucopyrus, FTIR, DLS, Nanoparticles. REFERENCES Chen, X., Schluesener, H.J., 2008. Nanosilver: a nanoproduct in medical application. Toxicol. Lett. 176, 1–12. Ahmed, S., Ahmad, M., Swami, B.L., Ikram, S., 2016. A review on plants extract mediated synthesis of silver nanoparticles for antimicrobial applications: a green expertise. J. of Adv. Res. 7, 17–28. Wang, D., Astruc, D., 2014. Magnetically recoverable ruthenium catalysts in organic synthesis. Molecules 19, 4635–4653. Iqbal, M., Usanase, G., Oulmi, K., Aberkane, F., Bendaikha, T., Fessi, H., Zine, N., Agusti, G., Errachid, E.S., Elaissari, A., 2016. Preparation of gold nanoparticles and determination of their particles size via different methods. Mat. Res. Bull. 79, 97–104. Rad, A.G., Abbasi, H., Afzali, H.M., 2011. Gold nanoparticles: synthesising, characterizing and reviewing novel application in recent years. Phys. Procedia 22, 203–208. Li, J., Li, Q., Ma, X., Tian, B., Li, T., Yu, J., Dai, S., Weng, Y., Hua, Y., 2016. Biosynthesis of gold nanoparticles by the extreme bacterium Deinococcus radiodurans and an evaluation of their antibacterial properties. Int. J. Nanomed. 11, 5931–5944. Keshavamurthy, M., Srinath, B.S., Ravishankar Rai, V., 2018. Phytochemicals mediated green synthesis of gold nanoparticles using PterocarpusSantalinus L. (Red Sanders) bark extract and their antimicrobial properties. Particul. Sci. and Tech. 36, 785–790. Sheny, D.S., Mathew, J., Philip, D., 2011. Phytosynthesis of Au, Ag and Au–Ag bimetallic nanoparticles using aqueous extract and dried leaf of Anacardium occidentale. Spectrochim. Acta A Mol. Biomol. Spectrosc. 79, 254–262. Manisha R Dondaa , Karunakar Rao Kudlea , Jahnavi Alwalaa , Anila Miryalaa , B Sreedharb and MP Pratap Rudra, Synthesis of silver nanoparticles using extracts of Securinega leucopyrus and evaluation of its antibacterial activity, ISSN 2250-1770, INT J CURR SCI 2013, 7: E 1-8. Kim, J.S., Kuk, E., Yu, K.N., Kim, J.H., Park, S.J., Lee, H.J., Kim, S.H., Park, Y.K., Park, Y.H., Hwang, C.Y., Kim, Y.K., Lee, Y.S., Jeong, D.H., Cho, M.H., 2007. Antimicrobial effects of silver nanoparticles. Nanomedicine 3, 95–101. Evelyn, M.L.P., Fernando, W.C., Araujo, I.A., Souza, I.V.G.A., Bastos, T.G., Silva, S.C., Nascimento, G.C.G., Militaao, L.A.L., Soares, H.S., Xavier, S.J.M., 2012. Pharmacological screening and acute toxicity of bark roots of Guettardaplatypoda. Braz. J. Pharmacogn. 22, 1315–1322. Ghosh, S., Patil, S., Ahire, M., Kitture, R., Gurav, D.D., Jabgunde, A.M., Kale, S., Pardesi, K., Shinde, V., Bellare, J., Dhavale, D.D., Chopade, B.A., 2012. Gnidiaglauca flower extract mediated synthesis of gold nanoparticles and evaluation of its chemocatalytic potential. J. Nanobiotechnol. 10, 1–9. Patil, M.P., Jin, X., Simeon, N.C., Palma, J., 2018. Anticancer activity of Sasa borealis leaf extract-mediated gold nanoparticles. Artif. Cells Nanomed. Biotechnol. 46, 82–88. Patil, M.P., Ngabire, D., Pham Thi, H.H., Kim, M.D., Kim, G.D., 2016. Eco-friendly synthesis of gold nanoparticles and evaluation of their cytotoxic activity on cancer cells. J. clust Sci. 16, 1051–1056. Huang, S.L., Weng, Y.M., Chiou, R.Y., 2001. Survival of Staphylococcus aureus and Escherichia coli as affected by ethanol and NaCl. J. Food Prot. 64, 546–550. Arokiyaraj, S., Vincent, S., Saravanan, M., Lee, Y., Oh, Y.K., Kim, K.H., 2017. 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Patil, M.P., Jin, X., Simeon, N.C., Palma, J., 2018. Anticancer activity of Sasa borealis leaf extract-mediated gold nanoparticles. Artif. Cells Nanomed. Biotechnol. 46, 82–88. Sathishkumar, M., Pavagadhi, S., Mahadevan, A., Balasubramanian, R., 2015. Biosynthesis of gold nanoparticles and related cytotoxicity evaluation using A549 cells. Ecotoxicol. Environ. Saf. 114, 232–240. Vijayan R, Joseph S, Mathew B. Anticancer, antimicrobial, antioxi-dant, and catalytic activities of green-synthesized silver and goldnanoparticles usingBauhiniapurpurealeaf extract. BioprocessBiosyst Eng. 2019;42(2):305–319. Poojari, S., Porika, R., & Mamidala, E. (2014). Phytochemical analysis and in vitro antidiabetic activities of Physalis angulata fruit extracts. Natl. J. Integr. Res. Med, 5, 34-38.

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Antibacterial, Securinega leucopyrus, FTIR, DLS, Nanoparticles

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selected citations
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This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
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influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
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