Zenodo deposit for Pozzi et al (2023) Local adaptation in prairie legume-rhizobia symbiosis Evidence of local adaptation to environment and nitrogen fixing rhizobia differs for two species of the prairie clover, Dalea candida and Dalea purpurea. Adrien CM Pozzi1,2, Ruth Shaw1, Georgiana May1,3 1 Department of Ecology, Evolution and Behavior, University of Minnesota Twin-Cities, St Paul, MN 55108. 2 Current affiliation: Université de Lyon, UMR Ecologie Microbienne Lyon (LEM), CNRS 5557, INRAE 1418, Université Claude Bernard Lyon 1, VetAgro Sup, France. ORCID: 0000-0001-6765-4293. 3 Correspondance: Georgiana May (gmay@umn.edu) Keywords: anthropogenic climate change; conservation; Dalea species; habitat fragmentation; local adaptation; mutualism; nitrogen-fixing symbiosis; population variation; prairie legume; rhizobia Description: This Zenodo deposit contains the following files: “Metadata & data” spreadsheet. Contains metadata and data about the Twin Valley experiment (including the position of plants, an intermediary census, growth and nodulation traits for harvested plants, data on bacterial isolates from root nodules, and source modifiers for GenBank accessions OQ732394-OQ732572). “Blast alignments output.txt” text file. Contains ouput of Blastn alignements for the 16S rRNA genes of bacterial isolates against the rRNA_typestrains/16S_ribosomal_RNA 16S ribosomal RNA (Bacteria and Archaea type strains) database, to determine the genus as recommended by GenBank during sequence submission. “General script.R”. R script of the general statistical analyses produced for publication. “General environment.RData”. The companion RData (environment) of the above R script. “Trait model script.R”. R script of the plant trait models produced for publication “Trait model environment.RData”. The companion RData (environment) of the above R script. Acknowledgments: The authors thank members of the May Lab (Kane Keller, Cedric Ndinga-Muniania, Emma Daily, Mai Beauclaire, Mara Demers, Liam Vertal, Monica Watson) for their help in field and lab work. We thank members of the Healthy Prairies project (Shelby Flint, Anna Peschel, Bill Peterson) who provided much of infrastructure with which this project was possible, as well as help in setting up field experiments. We also thank volunteer undergrads from University of Minnesota Morris (Amelia Nelson, Emily Job, Lily Fulton) for assistance in measuring harvested plants and counting nodules. Funding was obtained from MN LCCMR (Project 00086965, Healthy Prairies).

Part of the MN LCCMR Healthy Prairies project (00086965) granted to R. Shaw and G. May, UMN Twin-Cities.