Objectives: Tobacco smoke is one source of free radicals that can enter the body through the respiratory tract and circulate through the bloodstream to reach all organs in the body. Purple sweet potato (Ipomoea batatas L.) anthocyanin is a potentially powerful antioxidant that reduces free radicals. This study aims to demonstrate that purple sweet potato anthocyanin can reduce mammary gland malondialdehyde concentrations in female white rats (Rattus norvegicus) exposed to tobacco smoke. Methods: This was experimental research with a randomized post-test control group. Thirty female white rats were used in this study. These samples were then divided into two control groups (negative control group - no exposure to tobacco smoke and anthocyanin; positive control group - exposure to tobacco smoke only -) and three exposure groups of exposure to cigarette smoke at a rate of two sticks/day and variety Anthocyanin doses for eight weeks. The malondialdehyde content of the mammary gland was measured spectrophotometrically. Results: The result showed that the higher dose of Ipomoea batatas L anthocyanin administered, the lower the MDA level in the breasts of rats exposed to cigarette smoke (ANOVA test, p-value = 0.002) Conclusions: Ipomoea batatas L. anthocyanin can decrease the concentration of mammary gland malondialdehyde in female rats exposed to tobacco smoke.