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Pranayama is the best way to control energy through breathing. It works instantly & relieves stress and boosts energy. Regular pranayama practice is refreshing and balances emotions. Pranayama is scientifically proved tool to reduce the anxiety level and negative emotions. The aim of the study was to assess the effectiveness of the Pranayama on reducing Stress among antenatal mothers. A total 40 antenatal mothers were studied. The duration of the study was 4 weeks, and change outcomes were measured at the beginning & at the end of the study with help of questionnaire. Pre-test mean and SD were 62.45 & 11.46 respectively where as post test mean and SD were 32.90 & 7.90, with Chi-value 65.1538 and tabulated value at 0.0001. These reading indicated the effectiveness of Pranayama on Stress among antenatal mothers.