These DADA2-formatted training fasta files were derived from the Ribosomal Database Project's Training Set 16 and the 11.5 release of the RDP database. These fastas were generated by the following commands (using the dada2 R package version 1.5.1): path <- "~/Desktop/RDP/RDPClassifier_16S_trainsetNo16_rawtrainingdata" dada2:::makeTaxonomyFasta_RDP(file.path(path, "trainset16_022016.fa"), file.path(path, "trainset16_db_taxid.txt"), "~/tax/rdp_train_set_16.fa.gz") dada2:::makeSpeciesFasta_RDP("~/Desktop/RDP/current_Bacteria_unaligned.fa", "~/tax/rdp_species_assignment_16.fa.gz")