Introduction: Breast Imaging Reporting and Data System (BI-RADS) is the universal standard developed bythe American College of Radiology to characterize breast lesions on mammography.Histopathological assessment is usually doneaftersurgery,helpstocorrelatetheBI-RADSscoringsystem.).Molecular markers including estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor 2(Her2) have prognostic and predictive values and hence can be used to recommend treatmentguidelines. Methodology: A 2-year prospective cross-sectional observational study was conductedin the Departments of Pathology and Radiodiagnosis, Christian Medical College, Ludhiana. Atotalof62caseswhichhadundergonemammographyfollowedbyabreastbiopsywereincludedinthe study. Mammograms and Histopathology slides of all cases were reviewed independently by tworadiologists and pathologists respectively. Results: Lesions with smaller diameters on mammography and biopsy showed a stroke positive correlation (r =0.5461,p=0.0007). Significant associations of biomarkers with mammography characteristics were observed(ER with architectural distortion, p=0.005 ER with margins, p=0.005 PR with architecturaldistortion,p=0.009PRwithmargins,p=0.005Her2witharchitecturaldistortion,p=0.007Her2with margins, p=0.006). Conclusion: Significant associations between histopathological biomarkers and mammography characteristics could provide additional information at the first contact of a patient with a breast lesion.