Biofilm forming Methicillin-resistant Staphylococcus aureus (MRSA) strain remains a leading cause of infections with high frequency of morbidity and mortality in wound patient, but little information exists regarding the prevalence and characterization of biofilm forming MRSA from different wound sources. Thus, this study was aimed at determining the biofilm forming potentials of MRSA isolated from patient wound in National Orthopedic Hospital Enugu (NOHE) and Enugu State University Teaching Hospital (ESUTH) Enugu State. A total of 100 samples were collected from NOHE (76 from accident wound and 24 from burn wound) while 100 samples were collected from ESUTH (69 from accident wound and 31 from burn wound). The collected samples were analyzed and isolates identified using standard microbiological techniques. Antimicrobial susceptibility testing was carried out using Kirby–Bauer disk diffusion method and S. aureus isolates were screened for MRSA strain using cefoxitin and oxacillin. MRSA strains were studied for biofilm forming potential using Quantitative Assay. Multiple antibiotic resistance indices (MARI) were also determined. A total of 76(76.0 %) and 69(69.0 %) of S. aureus isolates were obtained from patients wound in NOHE and ESUTH respectively. Various degrees of resistance were observed among the S. aureus isolated to the tested antibiotics which ranged between 33.3 to 100 percentages. Exactly, 62(62.0 %) and 48(48.0 %) MRSA were found from patients wound in NOHE and ESUTH respectively. The prevalence of biofilm forming MRSA recorded 20(20.0 %) and 14(14.0 %) from patients wound in NOHE and ESUTH respectively. The isolates displayed average MARI value of ≥0.5. This study observed that biofilm forming MRSA can be treated with ciprofloxacin and imipenem. Thus, proper drug usage in the treatments of infection is recommended. Keywords: MRSA - Methicillin Resistance Staphylococcus aureus MSSA - Methicillin Susceptible Staphylococcus Aureus NOHE - National Orthopedic Hospital Enugu ESUTH - Enugu State University Teaching Hospital MARI - Multiple Antibiotics Resistance Index