Fungal infections are a major health concern and require effective treatments to prevent their spread. Natural products have shown promise as alternative treatments, with Ajmoda oil being one such product. In this review article, we discuss the formulation of an antifungal cream using Ajmoda oil and the preformulation studies and evaluation tests. The cream was formulated using a combination of Ajmoda oil, emulsifying wax, stearic acid, and distilled water. Preformulation studies were conducted to ensure the stability, compatibility, and rheological properties of the cream. The evaluation tests included physical appearance, pH, viscosity, spreadability, drug content, in vitro release, and antifungal activity. The results showed that the cream was stable, compatible, and had desirable rheological properties. The physical appearance was homogenous, and the pH was within the acceptable range. The viscosity and spreadability were optimal, and the drug content was consistent. In vitro release studies showed that the cream released the drug over a period of 24 hours. Antifungal activity studies demonstrated that the cream was effective against Candida albicans, Aspergillus niger, and Trichophyton rubrum. Thus, Ajmoda oil-based antifungal cream has great potential as a natural and effective treatment for fungal infections. Keywords – fungal infection, Ajmoda oil, Candida Albicans ,Cream, Natural.