Introduction: Urinary tract infections (UTIs) are amongst the most common pathological conditions in community and hospital environments. The bacterium Escherichia coli is its main etiological agent. The conventional medical treatment includes antibiotics; however, it is necessary to search for strategies that can avoid microbial resistance. This highlights the importance of Medicinal Biomagnetism (MB), a therapeutic system developed in 1988 by the Mexican physician Dr. Isaac Goiz Durán (1941-2021), which consists of the use of magnets in pairs, with magnetic flux greater than 1,000 Gauss, in topographic points that present biomagnetic resonance and aims to correct distortions in the body’s pH, helping to restore and maintain the Normal Energy Level (NEL). Objective: To evaluate people with UTIs caused by E. coli who were treated with MB. Methodology: MB treatment was carried out in two female participants (47 and 50 years old) and one male participant (54 years old), who had positive urine culture results for E. coli. The participant also had a high dose of total prostate specific antigen (PSA). Only the first case required a second MB session. Laboratory tests were repeated. Results and discussion: The urine culture tests indicated that there was no bacterial growth in cases treated with MB. And, the dosage of total PSA, indicated a reduction from 6.13 to 0.54 ng/mL. This study demonstrated that people showed elimination of UTIs caused by E. coli when treated with MB. It is suggested that this technique be researched in larger trials to better determine its contribution to the treatment of UTIs.