These datasets support the data provided directly in the scientific publication titled "Eggsplorer: a rapid plant–insect resistance determination tool using an automated whitefly egg quantification algorithm", published in the BMC Plant Methods journal. The figures and tables included in the datasets highlight various materials and methods employed to build a proof-of-concept of the Eggsplorer tool. These included whitefly assays, leaf image acquisitions, an automatic egg quantification algorithm, image pre-processing, object detection and image stitching, detection post-processing, algorithm evaluation and software programming. The provided figures and tables in the datasets are further discussed and interpreted in detail, as well as their subsequent results, in the scientific publication. This research was conducted within the VIRTIGATION project, which is part of the EU Open Research Data pilot. This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No. 101000570.