Figure S1. Phylogenetic tree original of Trichomycterinae based on 566 cytochrome b sequences (405 haplotypes) with 999bp. Bayesian Inference with values above each branch correspond to the branch consistency index (BPP); Figure S2. Phylogenetic tree original of Trichomycterinae based on 566 cytochrome b sequences (405 haplotypes) with 999bp. Bayesian Inference with values above each branch correspond to age for clades, as well as the margin of error demonstrated in node bars; Figure S3. Phylogenetic tree original of Trichomycterinae based on 566 cytochrome b sequences (405 haplotypes) with 999bp. Maximum Likelihood with values above each branch correspond to the branch consistency index (ML bootstrap); Table S1: Sequences used in the present study with their respective locations, references, haplotypes, and clades according to the phylogenetic tree in figure 2; Table S2: Type locality of the species nominally allocated to Trichomycterus and of the type species of the nine genera of Trichomycterinae. Type species in asterisk.; Table S3A: Table with percentages of K2P pairwise genetic distances between the subclades of Trichomycterus sensu stricto. Values in diagonal (below, dark grey) correspond to the genetic distance between clades by category. Values above (above, blue) refer to the respective standard errors. Bold values on the diagonal refer to the intraclade distance (1 to 31, as referenced in Table S1 and S3C); Table S3B: Genetic divergence range, by category, and occurrence within subclades. genera and subfamily; Table S3C: List of Alphabetical clade code and respective genus/species and numerical subclade code with respective species.