This work focuses on the phytochemical study and biological activity (antimicrobial) of the aerial parts of the Artemisia campestris collected from the Ahaggar National Park in southern Algeria. After extraction of the essential oil, chromatographic and biological analyzes were carried out on this species. The results show an average essential oil yield of 0.6±0.02 ml per 100 g of dry plant. Chromatographic analysis of the essential oil of Artemisia campestris subsp. glutinosa is rich in monoterpene compounds (50.47%), particularly in hydrogenated forms (40.24% of α and β-pinene) and sesquiterpenes (35.75% of Spathulenol (8.47%), β-Eudesmol (4.67%), and Carvomenthene (3.37% )... The study of antimicrobial activity revealed a remarkable antifungal activity for Candida albicans that could be exploited therapeutically.