The use of endoscopy and cytology for the diagnosis of aspergillosis has gained significant attention in recent years due to its accuracy and reliability. The present literature review highlights the potential advantages of combining these two techniques in diagnosing aspergillosis, including the ability to visualize affected tissues and detect the presence of fungal elements. The use of endoscopy and cytology is applicable both in Human and Avian medicine which provides a rapid diagnosis, earlier treatment, and improved patient outcomes, making these techniques valuable to diagnose aspergillosis.