In this study, a total of 65 neophytes (alien species introduced after 1500 A.D.), belonging to 51 genera and 25 families, occurring in the Czech Republic, were used. The species set represented plant families only from eudicots with Asteraceae, Brassicaceae Amaranthaceae, Polygonaceae and Fabaceae as the major families having 4 or more species. We measured different root morphological and biochemical traits (including exudate profiles) of these plant species grown in a controlled system.