Context:Alcoholism is characterized by development of dependence and withdrawal. The liver enzymes, especially GGT and MCV have beenwidelyusedaspotentialmarkers to quantify severity. Aims:To study clinical profile and biochemical parameters in patients with alcohol dependence syndrome attending tertiary hospital of Imphal. Settings and Design:Department of Psychiatry, RIMS, Imphal, it was a cross sectional study. Methods and Material:180 participants diagnosed with alcohol dependence syndromeas per ICD-10 criteria were included. Data obtained was recorded in semi-structured pro-forma. Statistical analysis used: IBM SPSS version 21 Results: Most patients had tremulousness (89%), and were oriented (75%). Most had tachycardia (79.4%). AST was raised in 92.8% of patients with mean value of198.05 IU/L, and ALT was raised in 89.4% of patients with mean value of 98.53IU/L. GGT was high in 93.9% of patients with mean value of 638.87IU/L. MCV was normal in most of the patients having mean value of 97.3 fl. Conclusions: Most participants diagnosed with alcohol dependence syndrome in this study had withdrawal symptoms in form of tremulousness and tachycardia, liver enzymes were significantly high in most of them. MCV was normal for most of the patients.