Data includes amino acid (µg AA mg DW-1) and phospholipid fatty acid content (µg FA mg DW-1) of the microbiome, mixotrophic algae, zooplankton, and fish from the four-trophic level experiment. The experiment included control (no addition), 13.5% 13C-labelled beech leaves (Fagus sylvatica), 97% 13C-labelled lignin-hemicellulose extracted from wheat (Triticum aestivum, ~80% lignin, 13% hemicellulose), and 99% 13C-labelled polystyrene (microplastic). Incubation time in humic lake water was 14 days in the control, leaf, and lignin experiment but 56 days for polystyrene, which after mixotrophic algae (Cryptomonas sp.) was introduced to the bottles. In the next step, herbivorous zooplankton (Daphnia magna) consumed microbes, mixotrophic algae, and particles for five days which after they were used as the diet to zebrafish (Danio rerio) during a five-day experiment.

This data has been used for a paper in Ecology Letters titled "The second life of terrestrial and plastic carbon as nutritionally valuable food for aquatic consumers".