Abstract Introduction: Today, drugs are being tried with various methods for the early healing of burn wounds. However, no drug still shows the desired effect in burn healing. Objective: This experimental study investigated the effects of platelet-rich plasma (PRP) and epidermal growth factor(EGF) on burn wounds. Method: In this study, 48 Spraque-Dawley male rats aged 12 to 16 weeks weighing 350 – 450 g were used under general anesthesia. The rats in Group 1 were in the control group, and no application was made. Only EGF was applied to the burn area created on the backs of the rats in the 2nd group, EGF + PRP was applied to the burn area created on the backs of the rats in the 3rd group, and only PRP was applied to the burn area created on the backs of the rats in the 4th group. Results: In terms of epidermis regeneration, there was a statistically significant difference between days 7, 14, and 21 of Groups 1, 3, and 4. Regarding granulation, there was a statistically significant difference between days 7, 14, and 21 of Groups 1, 2, and 3. Regarding inflammation, Groups 1, 2, and 4 are investigated seventh, 14th, and 21st days. There was a statistically significant difference between his days. In terms of hair follicle regeneration, there was a statistically significant difference between days 7, 14, and 21 of Groups 2, 3, and 4. Conclusion: It was concluded that platelet-rich plasma and epidermal growth factor applied by intralesional and perilesional injection methods positively affected wound healing and accelerated wound healing in rats with third-degree burns.