Liposomes are derived from Greek word lipo means fat and soma means body, composition of phospholipids these are made up of phospholipids bi layer, encapsulated in both hydrophilic and lipophilic drugs and safe guard from decoration these formulations due to its biodegradable and biocompatible characteristics, many drug delivery applications have been fully examined over the last decade. Acyclovir is BCS Class Ⅲ drug with low permeability. To enhance the permeability of acyclovir drug by liposomal drug delivery system by thin film lipid hydration by hand shake method. Acyclovir liposomes are penetrated to biological membrane and enhance their pharmacological action and drug release. Enhance the rate of drug permeability is enhanced there by increases the Bioavailability of poorly BCS class Ⅲ drug – Acyclovir and improved therapeutic efficacy and compliance of the patient. Recent trends so many students and research scholars desire and signifance will increase for this technology mainly because of wide range of therapeutic applications in targeting delivery of several enzymes’ antibiotics anti virals,anti-parasite transdermal transports and diagnostic tools and vaccines. The goal of topical liposome delivery approaches is to get access by combining different polymers.