The trained nnUnet models for Whole Regional Lymph Node Area Delineation with Deep Learning Model for Total Marrow and Lymphoid Irradiation. We are submitting the article, and we will update the information. ******** Abstract ******** Purpose Total body irradiation (TBI) has been performed for conditioning before hematopoietic stem cell transplantation. However, TBI can be related to diverse adverse events including radiation pneumonitis and cataract. Efforts to reduce these events include the total marrow irradiation (TMI) and total marrow and lymphoid irradiation (TMLI). Compared to TMI, TMLI requires more target delineations with lymph nodes which can be labor-intensive and time-consuming. However, with the TMI plans, the coverage to lymph node might be lower than TMLI and its clinical significance is unknown. In the current study, we aimed to develop a deep learning model for automatic delineation of whole regional lymph nodes and assess the dose coverage of lymph nodes with TMI plans using the model. Materials and Methods Whole regional lymph nodes (cervical, axillary, mediastinal, para-aortic, common iliac, external iliac, internal iliac, obturator, presacral, inguinal lymph nodes) were manually contoured and confirmed by 3 radiation oncologists in 26 patients having whole body computed tomography (CT) images. Twenty patients were designated as the training/validation set and 6 patients as the testing set, and model was developed using the 'nnUNET' framework. The trained model was evaluated with dice similiary coefficient (DSC), precision, recall and Hausdorff distance 95 (HD95). In addition, dose coverage of the manually delineated lymph nodes in TMI plans was calculated. Results The median value of DCS, precision, recall and HD95 of the trained model was 0.79 (IQR, 0.70-0.84), 0.83 (IQR, 0.75-0.89), 0.76 (IQR, 0.68-0.85), and 2.63 (IQR, 2.00-4.58) respectively. Dose parameters for manually delineated lymph nodes in previously treated TMI plans showed the median value of V100% (the percentage of volume receiving 100% of the prescribed dose), V95%, and V90% to be 46.0%, 62.1%, and 73.5%, respectively. The highest V100% was observed in presacral (71.1%), axillary (61.2%), obturator (60.3%), and internal iliac lymph nodes (84.67%). In contrast, the lowest V100% was identified in inguinal (13.3%), external iliac (21.8%), and cervical lymph nodes (42.1%). Conclusion Automatic delineation of lymph node using deep learning showed the potential to reduce the labor-intensive process of TMLI. When treated with TMI, the coverage of inguinal, external iliac, cervical lymph nodes was lower than expected.