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Conference object . 2019
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Evaluation of Various Primer Sets for the Real-time PCR Detection of Candidatus Liberibacter asiaticus causing Greening Disease in Citrus

Authors: Verlyn Palparan; Merahflor Cavite; Abbeah Mae Navasca; Benny Corcolon;

Evaluation of Various Primer Sets for the Real-time PCR Detection of Candidatus Liberibacter asiaticus causing Greening Disease in Citrus

Abstract

Citrus Greening Disease or Huanglongbing (HLB) is one of the most devastating diseases of citrus worldwide which is caused by an unculturable prokaryotic α-proteobacterium, Candidatus Liberibacter sp. Once the bacterium enters the tree, it propagates within the phloem producing die-back, yellow shoots, blotchy mottles on leaves, and off-tasting and malformed fruits (Bove, J. M., 2006). There are three (3) reported isolate types of bacterium namely, Candidatus Liberibacter asiaticus, Candidatus Liberibacter americanus, and Candidatus Liberibacter africanus (Li, W., et. al., 2007). In the Philippines, Candidatus Liberibacter asiaticus is most likely the species that is present among HLB-infected trees. With the alarming incidence of the disease and the fact that there is no available product that can combat the pathogen due to its nature, it is necessary to develop a reliable method that would accurately determine the presence of the bacterium for the integration on HLB management.

Keywords

Candidatus, qPCR, Citrus greening, Detection

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popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
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influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
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