Modifying the expression of cysteine protease gene PCP affects pollen develop-ment, germination and plant drought tolerance in maize

Figure S1: The sequencing results of pollen grains of T2 mutants. Figure S2: The relative expression levels of PCP were analyzed via RT-qPCR in pcp mutant lines. Data represent means ± SD of three replicates. Significant differences were indicated with different letters (P < 0.05, one-way ANOVA). Figure S3: Pictures of pollen germination and growth in vitro between WT and transgenic lines after 4 h and 6 h incubation. Scale bar: 100 μm; Figure S4: Phenotypic observation of WT and transgenic maize at flowering stage. Scale bar: 10 cm. Figure S5. Measurement of H2O2 content in WT and transgenic maize in response to drought stress by DAB staining. Table S1: PCR primers used in this study.

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