Prucalopride succinate is a selective 5-HT4 receptor agonist. Two simple UV spectrophotometric methods have been developed for the estimation of Prucalopride succinate in pharmaceutical dosage forms. The absorption maxima (λmax) of prucalopride succinate in Acetate buffer (PH-5.0) and 0.1N NaoH was found to be 222nm and 228nm respectively and Beer-Lambert’s law was obeyed over the concentration range 2- 16µg/ml. LOD and LOQ values of prucalopride succinate was found to be 0.143 µg/ml, 0.472 µg/ml and 0.112 µg/ml, 0.369 µg/ml in Acetate buffer and 0.1N NaoH respectively. The validation parameters were treated statistically with ‘t’ test and significant differences were noted. The study clearly revealed that the solvents influence the determination of Prucalopride succinate. The methods described were rapid and easy can be applied for the estimation of Prucalopride succinate in bulk and pharmaceutical formulations.