Black cumin (Nigella sativa) traditionally known as Antiurolithiatic legume in India, a representation of the family Ranunculaceae, is widely available. It is used as treat bronchitis, diarrhoea, rheumatism, asthma and skin disorder, digestive problems, tight parasitic infection, increase milk production in nursing mother, stops vomiting. Black cumin can be used as a comprehensive treatment in case of urolithiasis. Till date the molecular mechanism of Antiurolithiatic activity of black cumin is not clearly known. From the protein data bank the crystals structure of Adenine phosphoriboxyl transferase (PDB ID: 1L1Q), Glycolate oxidase (PDB ID: 2YVS), Oxalate oxidase (PDB ID: 2ETE) were acquired. Molecular docking of active component (Rutin, Quercine, Kaempferol) of Nigella sativa with Urolithiatic including enzyme shown significal reduction and change in preparation of urolithiatic activity. This investigation proved that Nigella sativa has potential to cure kidney stone. This present investigation will be important information in the field of urolithiatic. This is first hand report on in-silico analysis of antiurolithiatic activity of Nigella sativa. The outcome of this research have tremendous scope as individual insights and including contribution to research inputs for researchers working on the field of in-vivo and in-vitro studies. Keywords: Nigella sativa, Adenine phosphoriboxyl transferase, Glycolate oxidase, Oxalate oxidase