Introduction: The possible spread of SARS-CoV-2 from humans to animals and vice versa has led to questions about the role of the environment in cross-species transmission. The virus may be able to spread via indirect environmental routes such as fomites or aerosols, but the transmission routes needs further clarification. Detection studies can help to provide valuable information on the presence of the virus in the environment. Especially since environmental factors such as temperature and humidity can impact the infectious nature of respiratory viruses. Aim: This report aims to define the persistence of infectious SARS-CoV-2 in various environmental matrices and discuss the relevance of detecting infectious SARS-CoV-2 in the environment. Objectives include to compile data from 1) field studies detecting infectious SARS-CoV-2 in the environment 2) experimental studies assessing the persistence of infectious SARS-CoV-2 over time 3) to compare data from PCR and cell culture to inform on risk assessment. Methods: In collaboration with partner institutes of the OHEJP, field-and experimental studies were shared on the detection of (infectious) SARS-CoV-2 in environmental matrices. Additional studies were retrieved from bibliographic databases. Data from the obtained studies were compiled according to the aim and objectives of this report. Findings: Infectious SARS-CoV-2 has been successfully detected in aerosols and fomites in indoor (healthcare) environments. In other settings, detection of infectious SARS-CoV-2 was not conducted or failed. Data from experimental studies showed that multiple (environmental) factors greatly impact the persistence of infectious SARS-CoV-2 in water, surfaces, food and aerosols. Comparison between viral RNA and infectious virus in (waste)water showed a considerable difference in persistence. Conclusion: Detection of infectious SARS-CoV-2 can help to better inform and evaluate the possible risks of indirect viral transmission between people and animals