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Confocoal microscopy images, CellProfiler excel files of Pearson's coefficients between TMEM55B/RILPL1 or p.Rab10/RILPL1 in R1441C MEF or VPS35[D620N] MEF cells, and prism files of graphed Pearson's coefficients for each condition, as seen in: Parkinson’s VPS35[D620N] retromer lysosomal dysfunction induces LRRK2 mediated lysosomal association of RILPL1 and TMEM55B. Confocal images: +cntrl(or +mli2)R1441CMEFTmem55568ex200_mycRILPL1488ExM Expansion microscopy images in R1441C MEFs in DMSO or MLi-2 conditions. The 561 channel is endogenous TMEM55b and the 488 channel is transfected myc-RILPL1, 405 channel is DAPI. cntrl(mli2 or nz)_prab(or pRAB)10568mycRILPL1488_01 Two replicates of images in R1441C MEFs in DMSO (cntrl), MLi-2, or Nocodazole. The 561 channel is endogenous TMEM55b and the 488 channel is transfected myc-RILPL1, 405 channel is DAPI. cntrl(mli2 or nz)_tmem(or TMEM)10568mycRILPL1488_01 Two replicates of images in R1441C MEFs in DMSO (cntrl), MLi-2, or Nocodazole. The 561 channel is endogenous TMEM55b and the 488 channel is transfected myc-RILPL1, 405 channel is DAPI. dmso(mli2 or nz)_vps35d620ntmem568rilpl1488-01 Two replicates of images in D620N VPS35 MEFs in DMSO, MLi-2, or Nocodazole. The 561 channel is endogenous TMEM55b and the 488 channel is transfected myc-RILPL1, 405 channel is DAPI. Excel pages and prism files: Raw output from CellProfiler pipeline of Expansion microscopy endogenous TMEM55b transfected mycRILPL1 R1441C MEF: R1441CExMTMEMRILPL1.xlsx; R1441CMEFExMTMEM55RILPL1.pzfx IF of pRab10 endogenous and mycRILPL1 transfected in R1441C MEF: R1441CpRab10RILPL1Correlation.xlsx; IF of TMEM55b endogenous and mycRILPL1 transfected in R1441C MEF: R1441CTMEM55bRILPL1Correlation.xlsx; TMEM55bRILPL1pRab10Pearsonscorrelations.pzfx IF of TMEM55b endogenous and mycRILPL1 transfected in D620N VPS35 MEF: VPS35D620NTMEMRILPL1Correlation.xlsx; VPS35D620NMEFTMEM55RILPL1Correlation.pzfx
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