This dataset is for “Fluorescence of solvent-extractable organics in sub-micrometer forest aerosols in Hokkaido, Japan” by Afsana et al. in Atmospheric Environment. The contents are described in the sheet “readme” of the Excel file named “Dataset_sonia2023323.xlsx”. The excitation–emission matrix (EEM) spectra of OA extracts in solutions were measured using a fluorescence spectrophotometer (FP-6600, JASCO) using a 1-cm path length quartz cell. The ranges of the excitation and emission wavelengths were 220–500 nm and 250–650 nm, respectively. drEEM toolbox (version 0.2.0) for MATLAB (http://www.models.life.ku.dk/dreem) was used to preprocess the EEMs and to perform parallel factor (PARAFAC) analysis. The details of the analysis of the mass concentrations and the ion groups of OA extracts were reported in Afsana et al. (2022). The details of the analysis of the mass concentrations of biogenic molecular tracers were reported in Müller et al. (2017). The dataset was obtained under the support by JSPS KAKENHI Grant Numbers JP19H04253 and JP25281002. Afsana, S., Zhou, R., Miyazaki, Y., Tachibana, E., Deshmukh, D. K., Kawamura, K., and Mochida, M.: Fluorescence of solvent-extractable organics in sub-micrometer forest aerosols in Hokkaido, Japan, Atmos. Environ. Afsana, S., Zhou, R., Miyazaki, Y., Tachibana, E., Deshmukh, D. K., Kawamura, K., and Mochida, M.: Abundance, chemical structure, and light absorption properties of humic-like substances (HULIS) and other organic fractions of forest aerosols in Hokkaido, Sci. Rep., 12, 14379, 2022. Müller, A., Miyazaki, Y., Tachibana, E., Kawamura. K., and Hiura, T.: Evidence of a reduction in cloud condensation nuclei activity of water-soluble aerosols caused by biogenic emissions in a cool-temperate forest, Sci. Rep., 7, 1−9, 2017.