These datasets highlight the invasive weeds S. elaeagnifolium, S. rostratum and S. nigrum as potential ToBRFV hosts, and support the data provided directly in the scientific publication titled "Solanum elaeagnifolium and S. rostratum as potential hosts of the tomato brown rugose fruit virus", published in the PLOS journal. The data was obtained through a series of viral inoculations and bioassays, serological tests for viral infections (i.e. ELISA), viral RNA extraction and reverse transcription (RT)-PCR, as well as quantitative RT-PCR (RT-qPCR). The methods are described directly in the scientific publication with indicators allowing for interoperability and reusability by fellow researchers. The provided datasets are discussed and interpreted in detail, as well as their subsequent results, in the scientific publication. This research was conducted within the VIRTIGATION project, which is part of the EU Open Research Data pilot. This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No. 101000570.