This dataset contains seasonal and annual mean spot stream temperatures for the six groundwater-fed and seven lake-fed streams at the Turkey Lakes Watershed. We have also included seasonal and annual scale hydroclimatic variables (air temperature, solar radiation, discharge, precipitation, ice on/off dates, and April 1st SWE). The Turkey Lakes Watershed is approximately 65 km northwest of Sault Ste. Marie, Ontario, Canada. Manual spot stream temperature measurements were made by field technicians visiting the catchment outlets as part of a routine water quality monitoring program at the TLW. The stream temperature data record extends from 1983 through 2018. The stream temperature record for stream 013 is 1986-2018. Hydroclimatic data is also 1983-2018, with the exception of lake ice data which ended in 2015. See the methods section of the associated publication for more details.