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Data for Whole-cell modeling of E. coli colonies enables quantification of single-cell heterogeneity in the antibiotic response

Authors: Skalnik, Christopher J.; Cheah, Sean; Yang, Michael; Mattheus Wolff; Spangler, Ryan K.; Talman, Lee; Morrison, Jerry H.; +3 Authors

Data for Whole-cell modeling of E. coli colonies enables quantification of single-cell heterogeneity in the antibiotic response

Abstract

Data from simulations used to generate the figures in the paper Whole-cell modeling of E. coli colonies enables quantification of single-cell heterogeneity in the antibiotic response. Extracted folder contains the following items: sim_dfs: a folder containing the CSV files that must be moved into the data folder of the vivarium-ecoli repository before using ecoli/analysis/antibiotics_colony/plot.py to generate any figures glc_10000_fluxome.csv: Each row represents a reaction in central carbon metabolism (in same order as listed in validation/ecoli/flat/toya_2010_central_carbon_fluxes.tsv). Each column represents a single time point for a single cell in a baseline glucose simulation (seed 10000). Each value is a flux given in units of mmol/L/hr. Provided as input to ecoli/analysis/centralCarbonMetabolism.py script to reproduce fluxome validation plot. glc_10000_proteome_avgs.csv: Each row represents a protein monomer (in same order as sim_data.translation.monomer_data["id"] where sim_data is reconstruction/sim_data/kb/validationData.cPickle). Each column represents a cell in a baseline glucose simulation (seed 10000). Each row represents a protein monomer. Each value represents the average count of a given protein monomer for a given cell. Provided as input to ecoli/analysis/proteinCountsValidation.py script to reproduce proteome validation plot. glc_10000_expressome.csv: Each column represents a gene (with the exception of the final two metadata columns: "Time" and "Agent ID"). Each row represents a specific cell (agent) at a specific time in a baseline glucose simulation (seed 10000). Each value represents the number of new RNA transcripts for a given gene in a given cell at a given time. Provided as input to data/subgen_gene_plots/generate_plots.py script to calculate number of sub-generational/exponential genes among all genes and antibiotic response genes.

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Keywords

whole-cell, E. coli, modeling, simulation, antibiotics

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selected citations
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This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
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influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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