MinION plasmid deep long read sequencing for sequence verification

We sequenced four plasmid constructs, each with a whole MinION flowcell, for use in developing and testing a sequence verification procedure. The resulting pipeline can sequence verify plasmid constructs, generating a consensus sequence with associated confidence of each base, adhering to strict acceptance criteria . The data contained herein are a subset of the complete data; 30 fast5 files for each plasmid, to be used as example data. datHL_001519: BCRxV.TF.1 datHL_001521: BCRxV.VSVG.1 datHL_001617: BCRxV.GagPolRev.1 datHL_001620: BCRxV.VSVG.1_mutant

Related Organizations

Canada's Michael Smith Genome Sciences Centre
Canada

Keywords

plasmid sequence verification, long-read sequencing, minion

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