One of the important problems in cotton breeding is the creation of high-yielding, fast-maturing varieties with high fiber yield and quality and their wide use in production. In the world, the cotton plant occupies a key place in the world textile industry. One of the major standards of seed quality is the 1000 grain weight, which is effective on sprouting, seed potential, seedling growth, and plant performance. Therefore, an experiment was run in the form of completely random blocks in 3 iterations to study the effects of the 1000 grain weight on the sprouting rate and seed potential of cotton different 1000 seed weight treatments were used in a standard sprouting test and accelerated aging test inside a germinating machine.