The main aim of the present work is Formulation and in-vitro characterization of the Itraconazole liposomal drug delivery. The objectives are liposomes are prepared by using film hydration technique by using rotary evaporator, For the preparation of liposomes cholesterol and cholins are used, to improve the bio availability of a drug, to enhance the patient compliance, Finally, completion of formulation the evaluation parameters were conducted. It was concluded that the optimized formulation F9, followed zero order release where the regression value was found to be 0.900It was also found that the drug was released by diffusion as the regression in Higuchi’s plot was 0.988. The ideal liposomes film showed upheld appearance of the medicine stood out from the relieved film containing the free prescription.The in vitro release energy of medicine from the liposomes suspension and liposomes film followed the Higuchi scattering model. Moreover, the in vivo study in bunnies showed in a general sense higher rate and level of ketoconazole ingestion from sublingual fast dissolving liposomes film appeared differently in relation to that from oral business tablets. Key words: Formulation, In-Vitro Characterization, Itraconazole Liposomal Drug Delivery