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Experimental 3D convex hull tiling of a wild moss specimen ( Rhytidiadelphus or Eurhynchium ?) embedded in 1% agarose Raw data consists of 18 tiles of varying number of Z stacks, depending on where the 3D convex hull points were determined. Images acquired on a Zeiss Lightsheet Z1 using a W Plan-Apochromat 20x/1.0 UV-VIS objective simultaneous dual camera acquisition of autofluorescence under 488nm and 561nm excitation. Original voxel sizes are (x,y,z) = (0.326, 0.326, 2.0) microns Data provided here are tiles fused using BigStitcher, downsampled to the following voxel sizes - Moss_Fused_Downsampled.tif: (4, 4, 24) microns - Moss_Fused_Downsampled_Anisotropy_2.tif: (12, 12, 24) microns Sample acquisition, sample preparation, imaging and stitching performed by Olivier Burri, at the Ecole Polytechnique Fédérale de Lausanne BioImaging and Optics Platform (EPFL - SV - PTECH - PTBIOP)
Lightsheet, BigStitcher, Stitching, Tiling, Moss
Lightsheet, BigStitcher, Stitching, Tiling, Moss
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