In this deliverable, the RRI framework for the NextGenProteins project is established. First, the objects and impacts of the NextGenProteins project are placed in relation to the concept of RRI. This is done by elaborating on the global grand challenges related to alternative proteins and the ethical, responsibility and sustainability dimensions therein. Next, a matrix consisting of the conceptual dimensions of the RRI framework for the project is rolled out. Finally, the process, actions and interactions that constitute the RRI framework in practice are presented. The RRI framework of the project is summarised and explained in the conceptual matrix. The dimensions of the matrix relate to the process, product, purpose and people of the research and innovation activities, whereas the components of the matrix consist of anticipation & analysis, reflection & reason, engagement & inclusion and act & responsiveness. Furthermore, the Societal Readiness Thinking Tool0F 1 , developed in the New HoRRIzon project2 , is applied to the R&I process of the NextGenProteins project. RRI approaches inform the project in various ways, for example through regular and ad hoc meetings with the executive board and work packages. The RRI framework is further discussed via webinars and workshops organised during the project. In addition to the internal RRI processes of the project, the responsibility issues related to alternative proteins are discussed together with societal stakeholders. Finally, the RRI framework also covers the developmental evaluations of the R&I processes in the middle of the project and in the end of the project. In summation, the process of applying the RRI framework in the NextGenProteins project is envisioned to be highly co-creative process between the project actors and the societal stakeholders. The aim of the process is to develop and ensure high quality R&I project and outcomes, which will be acceptable and desirable for the society and environment.