This research paper assesses the impact of collaborative transport strategies on last mile deliveries through a Physical Internet vision and the use of an urban digital twin. The study uses a simulated environment to analyze the effects of different transport and collaboration strategies on operational, economic, and environmental indicators. The results show that collaborative transport strategies can significantly improve last mile delivery performance, reducing delivery times, costs, and emissions. The paper concludes that the use of urban digital twin in combination with collaborative transport strategies can be an effective approach to optimize last mile deliveries and promote sustainable urban logistics. Also, the research provides valuable insights for urban planners and logistics companies looking to optimize last mile delivery operations in cities.