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Method Development and Validation of Indacaterol Maleate By RP-HPLC In Bulk and Pharmaceutical Dosage Form

Authors: Manali Katkade; Anagha Baviskar; Kotade, Kiran; Charushila Bhangale;

Method Development and Validation of Indacaterol Maleate By RP-HPLC In Bulk and Pharmaceutical Dosage Form

Abstract

A simple, sensitive and accurate RP-HPLC method has been developed for the determination of Indacaterol maleate in bulk and marketed formulation. The λmax of the Indacaterol maleate was found to be 260 nm in Methanol: water (90:10) pH 3.0. The method shows high sensitivity with linearity 10 to 50µg/ml (regression equation: y = 38835x + 278291; r2 = 0.9993). The various parameters according to ICH guidelines and USP are followed for validating and testing of this method. The Detection limit and quantitation limit were found to be 0.146 µg ml –1 and 0.442 µg ml–1 respectively. The results demonstrated that the procedure is accurate, specific and reproducible (RSD <2%), and also being simple, cheap and less time consuming and appropriate for the determination of Indacaterol maleate in bulk and Table dosage form.

Keywords

Indacaterol maleate, HPLC method, Method development, Validation

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selected citations
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This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
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