Supplementary FIgures: The following supporting information can be downloaded. Figure S1: Structure of SK-216. Figure S2: Concentration of PAI-1 protein in the culture medium. Figure S3: Evaluation of the knockdown efficiency of PAI-1. Figure S4; Analysis of the gene copy number of MET and HER2. Figure S5: The genes expression involved in TGF-β induced EMT pathway b in the microarray data. Figure S6: A comprehensive evaluation of mRNA expression via microarrays was performed for osimertinib-tolerant and vehicle control (A) H1975 and (B) PC-9 cells, and pathway analysis was performed. Figure S7: Flowchart of participants. Supplementary Methods: The following supporting information can be downloaded. Isolation and culture of cancer cells obtained from a patient. The primers used for Quantitative real time PCR (qRT-PCR). EGFR T790M, C797S mutation, gene copy number analyses. Western Blot figures: Pictures of the membrane of the Western blot corresponding to Figure 1E.