This dataset contains skin conductance responses (SCR), electrocardiogram (ECG), respiration, electromyogram (EMG), pupil size (PSR), and gaze coordinates measurements for 107 healthy individuals participating in a double-blind, placebo-controlled, randomized trial using a Pavlovian differential configural fear conditioning experiment with 3 sessions. The placebo group (n=54) includes 35 females and 19 males, aged 23.06 +/- 2.91 years; the minocycline (n=53) group includes 35 females and 18 males, aged 23.28 +/- 3.76 years. All participants were screened in a health check-up before session 1. CSs were five static room images presented full-screen, one of which served as CS+, coupled with US, and the other four as CS-. The US was a 0.5-s electric shock of 250 square pulses with a 10% duty cycle, delivered with a constant current stimulator on participants' dominant forearm through a pin-cathode/ring-anode configuration. SOA between the CS onset and the US was 7.0 s. Participants underwent the conditioning task with CSs (CS+ 83% reinforced) and US delivery in session 1 with 4 blocks and were tested in a recall/extinction task one week later in session 2 with 4 blocks, with CS and auditory startle probe (ST) delivered 7.0 s after CS onset via headphones (ca. 102 dB, 40 ms duration). No US was delivered during session 2. Session 3 (relearning) immediately followed session 2 and had the same structure as session 1 (CS+ coupled with US, 83% reinforcement) but contained only 2 blocks. Participants in the minocycline group received a single oral dosage of 200 mg minocycline around 120 min before the start of fear conditioning in session 1, while the placebo group received mannitol. No drug was administered in sessions 2 and 3. The ITI was drawn on each trial uniformly at random between 7.5 to 10.5 s. The blocks within each session were recorded in succession with self-paced breaks.