Many plant Species used in the treatment of different diseases. Plant derived active compound have played an important role in the development of clinically useful agents. Guizotia abyssinica and Linum usitatissimum plant seeds are used for many disease treatments. Aim of the present study is investigate the phytochemical analysis of Methanol, petroleum ether, Chloroform and Acetone extracts of linum usitatissimum and Guizotia abyssinica plants by spectrometry like UV, IR, NMR and Mass spectrometry. Qualitative analysis of phytochemical Screening reveals the presence of Phenol, Saponins, Alkaloids, Protein and Carbohydrates. Current research describes a simple, effective and reproducible Comparative phytochemical analysis of natural seeds.