The DFG Practical Guidelines on Digitisation provide a fundamental basis for DFG-funded digitisation projects in its “Digitisation and Indexing” programme: They formulate standards and contain information on organisational, methodological and technical issues in the context of digitising and indexing objects relevant for research. They thus make an important contribution towards the sustainability, accessibility and compatibility of funded projects and to the resulting infrastructure. This document is an updated version of the Practical Guidelines last published in 2016 by the DFG. It was developed in consultation with the DFG Head Office by a group of authors initiated by the NFDI consortium NFDI4Culture, the majority of whose members have long played a part in shaping the Practical Guidelines and are actively involved in the NFDI consortia NFDI4Culture, NFDI4Memory, NFDI4Objects and Text+. The now revised Practical Guidelines on Digitisation serve as starting point for a material- and community-related differentiation of the Practical Guidelines by the communities. All communities and institutions concerned with the digitisation of research-relevant objects are encouraged to contribute their expertise to the further process.