Many hepatoprotective herbal preparations have been recommended in alternative system of medicine for the treatment of hepatic disorders. No systemic study has been done on protective efficacy of Crossandra Undulaefolia belonging to family Acanthanceae to treat diseases. The Hydro-Ethanolic extracts of aerial parts of Crossandra Undulaefolias (ECU). The animals were weighed each and divided in group of five. Liver damage was achieved by injecting Paracetamol (2 g/kg). The treatment groups pretreated with ECU Group-IV (200mg/kg) and Group- V (400mg/kg). Silymar in was used as reference standard drug. At the end of 7 days, blood was collected, liver extracted, weighed, processed for histopathological assessments and alteration in the levels of biochemical markers of hepatic damage like serum transaminases (AST,ALT), alkaline phosphatase (ALP) , bilirubin cholesterol, high density lipoprotein (HDL) and tissue GSH were tested in all the groups. The ECU exhibited a significant hepatoprotective effect by lowering the elevated serum levels of AST, ALT, ALP, total and direct serum bilirubin, cholesterol and significantly (p<0.05) increased HDL and moderately increased total protein and albumin in a dose dependent manner These biochemical observations were supplemented by treatment of liver toxicity in traditional medicine. Histopathological examination of liver sections. These findings suggest the use of this plant for its Hepatoprotective effects.