The objective of the present study was to evaluate the synergistic antimicrobial and anti-tubercular activities of phyllanthus acidus Methanolic extract. Antibacterial study was carried out by plate hole diffusion or agar well diffusion assay to determine the growth inhibition of bacteria. Antifungal activity was performed by the use of Saubouraud dextrose agar medium (SDA). The synergistic activity study was calculated by means of cup plate method (Kirbauy bauer technique) using two wells in a plate. The Methanolic plant extract of Phyllanthus acidus (500μg/ml) was used in combination with Oxytetracycline (500μg/ml). Anti-tubercular assay was performed using Micro plate Alamar Blue Assay (MABA) using the suspension of Mycobacterium tuberculosis H37Rv strain. The concentrations of plant extract were used are 1000 μg/ml, 500μg/ml, 250μg/ml, 125 μg/ml, 62.5μg/ml. The medicinal plant appear to have a broad antimicrobial activity spectrum, they could be useful in antiseptic and disinfectant formulation as well as in anti-tubercular activity. Among the various micro organisms, the Methanolic extract was more active against Micrococcus flavum. In antifungal activity the Methanolic extract shows positive results for all fungus. The anti-tubercular activity was compared with standard drug Rifampicin. The Methanolic extract was having more percentage inhibition when compared to other extracts.