Uploaded zip-folder contains the following files: 1. A representative raw dataset of a 2D LSFM ventricular cardiomyocyte undergoing stimulated calcium transients and calcium sparks (frame_0000.tif -frame_17999.tif) 2. The recorded pacing signal time trace (waveform_test.xslx) 3. Image corresponding to the time-averaged background (AVG_19_35_39_LowNA rolling shutter.tif) 4. Pre-processed nuclear mask matrix (NuclearMask.mat), CMO-channel average (CMO_Average.mat), and CMO channel maximum intensity projection (CMO_MIP). 5. Split and co-registered data for each spectral channel (CMO_frame_00001.tif-CMO_frame_18000.tif, FLUO4_frame_00001.tif -FLUO4_frame_18000.tif). Compressed file size: 14.9 GB Uncompressed file size: 42.8 GB. Related to the following manuscript: Liuba Dvinskikh, Hugh Sparks, Ken MacLeod and Chris Dunsby " High-speed 2D light-sheet fluorescence microscopy enables quantification of spatially varying calcium dynamics in ventricular cardiomyocytes" (2023), In review with Frontiers in Physiology, Cardiac Electrophysiology.

This version has been updated to include processed split and co-registered data for each spectral channel.