Another investigative technique was set up for estimation of RP-HPLC strategy utilizing deferiprone cases. The chromatographic conditions were effectively created for the partition of Deferiprone by utilizing INERTSIL column, C18(150x4.6 ID) 5µm, stream rate 1.0ml/min, versatile stage proportion Triethylamine Buffer: ACN (50:50v/v), recognition frequency was 280nm. The maintenance time was seen as 4.9mins. The % virtue of deferiprone was seen as 99.08%. The framework appropriateness boundaries for deferiprone, for example, hypothetical plates and following elements were seen as 2567,1.512. The logical technique was approved according to ICH guidelines (ICH, Q2, (R1)). The linearity concentrate for Deferiprone was found in fixation scope of 125-375µg/ml and relationship coefficient were seen as 0.994. The % recuperation was seen as 98.40%. The %RSD was seen as 0.28. The accuracy study was precise, robust and repeatable. LOD esteem was 22.93µg/ml and LOQ esteem was 96.37µg/ml. As this strategy has shorter maintenance time and high goal, makes the technique more satisfactory and financially savvy and can be viably applied for routine examination in research establishments, quality control divisions in ventures and in clinical pharmacokinetic concentrates in not-so-distant future. Keywords: Deferiprone, RP‐HPLC, Method development, Validation